Photocleavable spacer

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WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any … WebSep 17, 2024 · In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed higher cytotoxicity as compared to RNA nanoparticles without the photocleavable spacer. The methodology provides proof of concept for the application of the light-triggered controlled release of drugs from RNA nanocages. …

Photocleavable PC Spacer Oligonucleotide Modification - Bio-Syn

WebPhotocleavable Modifications. Explore our photocleavable analogues of the Amino- and Spacer-Modifiers and the Biotin label, as well as our PC linker molecule. The general design of the PC monomers is based on an α-substituted 2-nitrobenzyl group. The photo-reactive group is derivatised as a cyanoethyl phosphoramidite that can be incorporated ... WebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would then be used to release the oligo, thereby decoupling antisense effects within the cytoplasm from antisense effects within the nucleus. how are nitrogen and phosphorus similar

Photocleavable Spacer Oligonucleotide Modification - Bio-Syn

WebPC Biotin Azide is an azide-activated photocleavable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to an azide group through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5 ... WebThe spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer … WebPC Linker (photocleavable) Spacer C3. Spacer C12. Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens), and is used to incorporate a spacer arm into an oligonucleotide. Spacer 9 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 9 has been used to form non-nucleotide bridges ... how many mg of benadryl is safe

PC Spacer (photocleavable) Oligo Modifications from Gene Link

Spacer Phosphoramidite 18 - Glen Research

http://genelink.com/newsite/products/mod_detail.asp?modid=134 WebFeb 5, 2010 · DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable ... how many mg of caffeine are in a bangWebAug 1, 1995 · While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm to a ... how are nitroglycerin pills taken

"WebIMAGING SPACERS. Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer … " - Photocleavable spacer

Photocleavable spacer

Glen Report 14.14 - PC Biotin and Related Photocleavable …

http://genelink.com/newsite/products/mod_detail.asp?modid=18 WebSpacer Modifiers. The hydrophilic spacers; Spacer 9 ( LK2128) and Spacer 18 (HEG) ( LK2129) are also particularly useful to build in flexibility within an oligo sequence. 6. As with 3’-‘spacers’ these are known to be nuclease …

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WebPC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide … WebUV-MALDI analysis reveals the result of the hybridization experiment along with the peptide fragments as photocleavable mass markers (PCMM). 7 By incorporating a …

WebPhotocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW ... WebThe light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA nanoparticles. Upon irradiation with ultraviolet light, cargos were rapidly released (within 5 min). In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed ...

WebBio-Synthesis offers photocleavable PC Biotin Oligo Modification at various scales. This light activatable PC biotin modified oligonucleotide contained a photocleavable spacer between the biotin group and the DNA bases. The biotin group is separated from the 5' end nucleotide base by the photo-cleavable group and a long chain spacer arm. WebPC Biotin Alkyne an azide-reactive photocleavable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to an azide group through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 ...

WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any substrate. In experiments using [leucine]-enkephalin as a model substrate, we show that PCB retains its high affinity toward avidin/streptavidin and allows rapid (< 5 min) and …

WebPhotocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon … how many mg of caffeine a day is safePhoto-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC Spacer is a 10-atom linker arm that can only be cleaved when exposed to UV light within the appropriate spectral range. The resulting oligo will have a 5’ phosphate group that is available for ... how are nike shoes producedhttp://genelink.com/oligo_modifications_reference/OMR_generate_pdf.asp?mod_sp_cat_id=22 how many mg of b12 per day for womenWebThe PC spacer (LK2131) and PC linker (LK2066) amidites can be incorporated within an oligonucleotide sequence. This is particularly useful in adding a modifier as a phosphoramidite where there is a need to remove this from the oligonucleotide at a later stage. ... Photocleavable Peptide-Oligonucleotide Conjugates for Protein Kinase Assays … how many mg of caffeine a day pregnantWebConveniently introduce spacer modifications into your oligonucleotide during synthesis with our high-quality spacer phosphoramidites and solid supports. Adding a spacer adds … how are nobel prizes fundedWebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would … how are nissan rogue ratedWebJan 6, 2003 · This unique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphoramidite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry ( 8 ... how are nj taxable wages calculated