T. thermophilus hb27
WebNov 4, 2015 · The thermophilic bacterium Thermus thermophilus (T. thermophilus) has high levels of lipolytic activity, and its whole genome has been sequenced. One esterase from the T. thermophilus strain HB27 has been widely characterized, both in its native form and in recombinant forms, being expressed in mesophilic microorganisms. WebRecent work has shown that DNA can be transferred by conjugation from T. thermophilus strain HB8 to a closely related strain, HB27. Strain HB8 harbors a region of its chromosomal DNA similar in sequence to the F plasmid of E. coli which allows conjugal transfer of chromosomal genes after it integrates into the chromosome of the donor cell.
T. thermophilus hb27
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WebWe cloned the genes encoding Mn-dependent catalases from Thermus thermophilus HB27 and HB8 and a less thermostable mutant carrying two amino acid replacements (M129V and E293G). When the wild-type and mutant genes were overexpressed in Escherichia coli, unmodified or six-His-tagged proteins of the expected size were overproduced as inactive … WebMay 5, 2024 · The wild-type DNA polymerase I gene from T. thermophilus HB27 (NCBI-protein ID: AAS81038) is 2505 nucleotides long with a GC content of 67.5% [14]. Although we first used this polymerase expressed in E. coli as a source of enzyme to amplify short and long target DNA sequences containing the polymerase gene itself, no amplification was …
Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebT. thermophilus HB27 wild-type cells as well as the ΔpilF::bleo mutant and complemented mutants expressing truncated versions of pilF were incubated with 5 µg DNA of a …
WebLaboratory-adapted strains of Thermus spp. have been shown to require oxygen for growth, including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of … WebThe pCT3FK shuttle fosmid carries additional selection and mobilization elements for the Thermus host, in particular a thermostable kanamycin resistance cassette and constitutive slp promoter (de Grado et al., 1999) and a sequence of the pyrE locus for chromosomal integration of the vector in T. thermophilus HB27 via
WebThermus thermophilus HB27 Taxonomy ID: 262724 (for references in articles please use NCBI:txid262724) current name. Thermus thermophilus HB27. equivalent: Thermus …
WebThermus thermophilus (T. thermophilus) HB27 is an extreme thermophile that grows optimally at 65-72 °C. Heat-induced DNA lesions are expected to occur at a higher … city jobs in indianapolisWebJan 1, 2012 · Mining the T. thermophilus HB27 genome resulted in the identification of a gene encoding a putative new short-chain alcohol dehydrogenase (Tt27-ADH2). Moreover, the protein encoded by such gene was 99% homologous to an alcohol dehydrogenase from T. thermophilus HB8 that have been crystallized (PDB code is 1UAY) but not … did bts perform at the billboard music awardsWeb[Methods in Molecular Biology] Lipases and Phospholipases Volume 861 Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27 city jobs in iowaWebBased on phylogenetic analysis of 16 S and 18 S rRNAs, the common ancestor of all organisms (Commonote) was proposed to be hyperthermophilic. We have previously tested this hypothesis using enzymes w did bts really disbandWebA Thermus thermophilus HB27 strain was constructed in which the malate dehydrogenase (mdh) gene was deleted. The Δmdh colonies are recognized by a small-colony phenotype. Wild-type phenotype is restored by transformation with Thermus plasmids or integration vector containing an intact mdh gene. The wild-type phenotype provides a positive … city jobs in laWebThermus thermophilus HB27 is an extremely thermophilic eubacteria with a high frequency of natural competence. This organism is therefore often used as a thermophilic model to … did bts perform at the grammysWebDec 6, 2024 · extracellular lipases from the Thermus thermophilus HB27 strain, which had molecular masses of 62 and 34 kDa, respectively [2]. T. thermophilus was found in hot springs at neutral and alkaline pH, as well as in hot water or in natural waters subjected to thermal pollution and was shown to grow optimally at temperatures around 65 ~ 75oC [22]. city jobs in lakeland fl